Suchergebnisse

The IV Russian International Conference on Cryo-Electron Microscopy (RICCEM-2023) was held at the Faculty of Biology of Lomonosov Moscow State University in a hybrid (offline and online) format and brought together more than 250 researchers from 10 countries. Following the conference, a special issue of the journal "Lomonosov Biology Journal" ("Vestnik Moskovskogo universiteta. Seria 16. Biologia") was published. This article briefly summarizes the main results of the conference and the content of the works published in the special issue.

In this work, conditions were selected for obtaining a sample of eukaryotic chaperonin TRiC
suitable for studying by cryo-electron microscopy. Using the method of differential scanning
(time-resolved) fluorimetry, the temperature stability of protein samples at different concentrations of salt and glycerol was compared, and then the selected conditions were used to prepare the
sample for microscopy. As a result, the structure of bovine TRiC in an open conformation was
obtained at 4.42 Å resolution.